Your research projects consist in identifying DNA fragments that have appeared or disappeared in a genome over time, or DNA point mutations.
The approach that best fits your needs will be proposed to you by the technical teams of BIOMNIGENE. To identify all mutations present within the genome, a resequencing by NGS will be proposed ; for mutations (point mutations or indel) in a targeted gene, a Sanger sequencing, restricted to a defined region of the genome, will be favored.
PCR and qPCR are two approaches that may be considered if the identification of the modified base(s) is not a major concern/is of little importance.