You study the mechanisms leading to the production of different messenger RNAs from a single gene and wish to define the coding regions of a gene.
BIOMNIGENE proposes several technical approaches such as RT-PCR, identification by fragment analysis and Sanger sequencing of the different mRNAs produced from a gene or sequencing by NGS of total RNA for a more global view of the splice variants of a gene.
Your needs
Genetic material
Total RNA
Total messenger RNA
Target mRNA(s)
Methods
De Novo sequencing
Re-sequening
Analysis of splice variants
Techniques
NGS
Sanger
RT-qPCR
