You want to compare/aim at comparing the expression level of one or more genes produced:
- by an organism incubated under different conditions or in different environments
- by different organisms grown under the same conditions.
In compliance with the principles of GLP (Good Laboratory Practice), BIOMNIGENE performs the reverse transcription of all messenger RNAs and the real-time PCR on cDNAs using primers specific to the gene(s) of interest. Expressed as ratios compared to referent gene (housekeeping genes), expression levels between different experimental conditions can be compared.
Your needs
Genetic material
Total RNA
Total messenger RNA
Target mRNA(s)
Methods
De Novo sequencing
Re-sequening
Analysis of splice variants
Techniques
NGS
Sanger
RT-qPCR
