You want to evaluate the impact of active substance or environmental factors on the expression of an individual's gene.
In order to evaluate/assess the changes that occurred during exposure to the molecule of interest, we can either determine the level of expression of a target gene by qPCR using cDNA obtained by reverse transcription of the RNA of interest, or perform random sequencing of the transcriptome by RNA-Seq.
The choice of method will be based on the number of genes to be studied.
Your needs
Genetic material
Total RNA
Total messenger RNA
Target mRNA(s)
Methods
De Novo sequencing
Re-sequening
Analysis of splice variants
Techniques
NGS
Sanger
RT-qPCR
